Solubilization of chick kidney microsomal cytochrome P -450
نویسندگان
چکیده
منابع مشابه
Purified Liver Microsomal NADPH-Cytochrome P-450 Reductase
NADPH-cytochrome P-450 reductase was isolated from liver microsomes of phenobarbital-induced rats. The enzyme exhibits an apparent minimal molecular weight of 76,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and contains 1 molecule each of FMN and FAD. Trypsin treatment of the reductase yields an enzyme with an apparent minimal molecular weight of 69,000 which r...
متن کاملPurified Liver Microsomal NADPH-Cytochrome P-450 Reductase
NADPH-cytochrome P-450 reductase was isolated from liver microsomes of phenobarbital-induced rats. The enzyme exhibits an apparent minimal molecular weight of 76,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and contains 1 molecule each of FMN and FAD. Trypsin treatment of the reductase yields an enzyme with an apparent minimal molecular weight of 69,000 which r...
متن کاملInteraction of liver microsomal cytochrome P-450 and NADPH-cytochrome P-450 reductase in the presence and absence of lipid.
Phospholipid has been reported to be necessary for optimal catalytic activity of a number of mammalian cytochrome P-450 (P-450) systems. We also confirm that a number of individual phospholipids and mixtures, used as soluble monomers or phospholipid vesicles, show activation of 7-ethoxycoumarin O-deethylase activity by an enzyme system composed of rat liver microsomal P-450PB-B and NADPH-P-450 ...
متن کاملSolubilization of cytochrome P-450 in high yield from Saccharomyces cerevisiae microsomal membranes by 1% Triton X-100: stabilization effect.
the high glucose concentrations or assisted excretion of cyclic AMP out through the cell membrane could account for the glucose effect in repressions of many enzymes, and on the production of cytochrome P-450. The subcellular location of adenylate cyclase in S . cerevisiae is dependent on the growth conditions of the yeast. Growth in 5% glucose-containing media was associated with the finding o...
متن کاملNADPH-cytochrome c reductase and its role in microsomal cytochrome P-450-dependent reactions.
The study of liver microsomal electron transport and drug metabolism activities involving NADPH-cytochrome c reductase has been performed using immunochem ical techniq ues ( I -4). This approach has been particularly valuable in establishing the role of this flavoprotein in the reduction of microsomal cytochrome P-450 and, thus, in the metabolism of drugs (2, 3). The existence of NADPH-cytochro...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1978
ISSN: 0014-5793
DOI: 10.1016/0014-5793(78)80565-1